Differentiation of Mesenchymal Stem Cell toward the Insulin-like Cells with Lentivirus Vector Mir-375

Background & Objective: Type1 diabetes is characterized by autoimmune destruction of pancreatic &beta; cells, leading to reduced insulin secretion. Differentiation of mesenchymal stem cells (MSCs) into &beta;-like cells offers new ways of diabetes treatment. MSCs can be insulated from the human umbilical cord tissue and differentiate into insulin-producing cells. Material & Methods: Human umbilical cord&ndash;derived stem cells (hUDSCs) were attained after birth, selected by plastic adhesion, and considered by flow cytometric analysis. For the differentiation induction, miR-375 was lentivirally over expressed in hUDSCs. Results: Meaningful that microRNAs (miRNAs) are the key players in several stages of pancreatic Differentiation, we have presented a novel and cost-effective strategy in which the over-expression of miR-375 promotes the pancreatic differentiation in hUDSCs in the absence of any other stimulator. The major expressions of PDX1 and insulin were identified by quantitative Reverse Transcription&nbsp;Polymerase Chain Reaction&nbsp;(RT-PCR) (P <0.05). Conclusion: In conclusion, the Morphological assessment and the expression analysis of islet marker genes demonstrated that hUDSCs are able to differentiate into insulin-producing cells by transduction with lentiviral vector miR-375